New Fee Schedule, as of July 1, 2007:
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A. Transmission Electron Microscopy |
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Full |
Independent** |
| Routine
Specimen Preparation:* |
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1)
fixation through embedding |
1-2
samples |
$350.00 |
$70.00 (for supplies only)
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3-10
samples |
$450.00 |
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over
10 samples |
+$45.00/each
additional sample |
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| Microtomy: |
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1)
thick sectioning & staining/blk |
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$35.00/blk |
$20.00/hr (Leica Ultracut only) |
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2)
thin sectioning & staining/blk |
(for every 4 grids ) |
$55.00/blk |
$20.00/hr
(Leica Ultracut only) |
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| Electron
Microscopy: |
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per hour spent on scope |
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$160.00
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$80.00
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| Photography:*** |
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1) Compact discs
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$4.00 |
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2) Prints on plain paper |
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$1.00 |
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3) Prints on glossy paper |
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$2.00 |
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| Technical
Time: |
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$80.00/hr |
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| *A
modified Karnovsky's fixative can be provided for up to 10 specimens,
additional fixatives or special types will be at an extra cost. Tissue specimens for
TEM should be about 2 x 2 x 2mm or smaller. For special types of
specimens (cultured cells, thin layers of tissue, tissue that requires
special orientation) contact Caroline Miller (camiller@anatomy.iupui.edu)
for instructions. |
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| **This
represents a reasonable amount of supplies for fixation and embedding, including, slides, grids, stains, and equipment usage. The EM Center provides 4 hours of training in electron microscopy techniques and use of the TEM . The cost for this training is charged to the researcher at the independent rate of $80/hour. |
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| ***This
represents direct digital images. EM film is not used in the EM Center |
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| B.
Special Procedures, Immunocytochemistry-EM level (no independent) |
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| Specimen Preperation: |
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1)
Fixation- embedding, unicryl |
1-2
samples |
$350.00 |
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3-10
samples |
$450.00 |
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over 10 samples |
+$45.00/sample |
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2)fixation-embedding, LRG |
1-2 samples |
$385.00 |
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3-10 samples |
$495.00 |
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over
10 samples |
+$50.00/sample |
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3) Cryo Preparation |
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a. cryo fixation |
1-2 samples |
$100.00 |
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over 2 samples |
+45.00/sample |
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b. high pressure freezing |
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$100.00/hour |
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This includes the cost of 1 technician, a minimum of 20 liters of liquid Nitrogen ($4/liter) and specific HPF supplies. |
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c.Automatic Freeze Substitution |
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$100.00/run |
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This includes the cost of a minimum of 20 liters of liquid Nitrogen ($4/liter) and specific AFS supplies. |
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Microtomy: |
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1)
Thick sectioning & staining/blk |
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$40.00 |
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2) Thin sectioning & staining/blk |
8 grids |
$60.00 |
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Eight grids represents enough for 2 immuno attempts, there will be a $5 charge per grid over eight. |
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3)Cryo sectioning/blk, thick and thin |
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$150.00 |
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There will be an additional charge for liquid Nitrogen ($4/liter) min of 40 liters |
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4)
Vibratome sectioning/sample |
$80.00 |
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5)
Immunostaining, for each attempt & antibody**** |
$400.00 |
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6)
Negative Staining (per grid) |
$45.00 |
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| ****Results
cannot be guaranteed since antibody specificity/titer, quality/fixation
of tissue and amount of target protein can limit success. Investigator
provides primary antibody and necessary background literature. |
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| C.
Scanning Electron Microscopy: |
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Full |
Independent** |
| Routine
Specimen Preparation: |
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1)
Fixation-coating, |
5 samples |
$500.00 |
$90.00 (supplies only) |
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over 5 samples |
+$45.00/each |
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| Electron
Microscopy: |
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$160.00/hour |
$80/hour |
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| Photography: |
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1) compact discs |
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$4.00
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2)prints on plain paper |
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$1.00 |
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3) prints on glossy paper |
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$2.00 |
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**This represents a reasonable amount of supplies for fixation through drying (CPD or chemical), mounting and coating. The EM Center provides 4 hours of training in electron microscopy techniques and use of the SEM. The cost of this training is charged to the researcher at the independent rate of $80/hour. |
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| D.
EM evaluation/consultation:
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| Each incoming project receives an hour consultation at no charge, thereafter there is an $80 per hour charge for time spent researching techniques, evaluating data (quantitive and/or qualitive) or for anything extra beyond routine. The EM Center provides 4 hours of training in electron microscopy techniques or use of either the TEM or SEM. The cost for this training is charged to the investigator at the independent rate of $80/hour. |
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| E. Outside/ Commercial Projects |
| Each project will be charged double the cost of regular fee schedule, due to lack of subsidy. |